例如:"lncRNA", "apoptosis", "WRKY"

The roles of oxidative stress and Beclin-1 in the autophagosome clearance impairment triggered by cardiac arrest.

Free Radic. Biol. Med.2019 May 20;136:87-95. Epub 2019 Apr 03
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摘要


During cardiac arrest and return of spontaneous circulation autophagosome clearance in the cortex is progressively impaired, but the role of reactive oxygen species in this process and the mechanism underlying the autophagy impairment remain unknown. In this study, we investigated the impacts of on the autophagy-lysosome pathway after in rats. Cortices from CA-duanyu1670C rats revealed accumulation of LC3, p62 and ubiquitin, indicating impaired autophagic flux. Furthermore, impairment of autophagic flux was related to lysosomal lesion, as indicated by decreased cathepsin D and lysosomal-associated membrane protein 2 (LAMP2) levels after In vitro, the resulting duanyu1670 generation blocked autophagosome processing and caused accumulation of LC3-II, ubiquitin, and p62, leading to mitochondrial dysfunction and cell death; this outcome was alleviated by cyclosporine A (CsA) pretreatment. Interestingly, ischemia/reperfusion injury was connected with Beclin-1 upregulation and a reduction in LAMP2, which is a pivotal protein in the autophagy-lysosome pathway. Recovery of the LAMP2 levels and partial Beclin-1 silencing restored the autophagic flux and reduced cell death after CA-duanyu1670C. Taken together, our data indicate that CA-duanyu1670C injury impairs autophagosome clearance partially through a decline in LAMP2 and increase in Beclin-1, leading to increased neuronal cell death.

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