Epithelial Expression of an Interstitial Lung Disease-Associated Mutation in Surfactant Protein-C Modulates Recruitment and Activation of Key Myeloid Cell Populations in Mice.

Patients with idiopathic pulmonary fibrosis (IPF) often experience precipitous deteriorations, termed "acute exacerbations" (AE), marked by diffuse alveolitis and altered gas exchange, resulting in a significant loss of lung function or mortality. The missense isoleucine to threonine substitution at position 73 (I73T) in the alveolar type 2 cell-restricted surfactant protein-C (SP-C) gene (SFTPC) has been linked to clinical IPF. To better understand the sequence of events that impact AE-IPF, we leveraged a murine model of inducible SP-C^I73T (SP-CFlp ) expression. Following administration of tamoxifen to 8-12-wk-old mice, an upregulation of Sftpc initiated a diffuse lung injury marked by increases in bronchoalveolar lavage fluid (BALF) protein and histochemical evidence of CD45⁺ and CD11b⁺ cell infiltrates. Flow cytometry of collagenase-digested lung cells revealed a transient, early reduction in SiglecF^hiCD11b^lowCD64^hiCD11c^hi macrophages, countered by the sequential accumulation of SiglecF^loCD11b⁺CD64^-CD11c^-CCR2⁺Ly6C⁺ immature macrophages (3 d), Ly6G⁺ neutrophils (7 d), and SiglecF^hiCD11b^hiCD11c^lo eosinophils (2 wk). By mRNA analysis, BALF cells demonstrated a time-dependent phenotypic shift from a proinflammatory (3 d) to an anti-inflammatory/profibrotic activation state, along with serial elaboration of monocyte and eosinophil recruitment factors. The i.v. administration of clodronate effectively reduced total BALF cell numbers, CCR2⁺ immature macrophages, and eosinophil influx while improving survival. In contrast, resident macrophage depletion from the intratracheal delivery of clodronate liposomes enhanced Sftpc -induced mortality. These results using Sftpc mice provide a detailed ontogeny for AE-IPF driven by alveolar epithelial dysfunction that induces a polycellular inflammation initiated by the early influx of proinflammatory CCR2⁺Ly6C^hi immature macrophages.

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