Effects of unpaired 1 gene overexpression on the lifespan of Drosophila melanogaster.

BACKGROUND:The signaling pathway is involved in many aging-related cellular functions. However, effects of overexpression of genes controlling JAK/duanyu1813 signal transduction on longevity of model organisms have not been studied. Here we evaluate the effect of overexpression of the unpaired 1 (upd1) gene, which encodes an activating ligand for JAK/duanyu1813 pathway, on the lifespan of Drosophila melanogaster. RESULTS:Overexpression of upd1 in the intestine caused a pronounced shortening of the median lifespan by 54.1-18.9%, and the age of 90% mortality by 40.9-19.1% in males and females, respectively. In fat body and in nervous system of male flies, an induction of upd1 overexpression increased the age of 90% mortality and median lifespan, respectively. An increase in upd1 expression enhanced mRNA levels of the JAK/duanyu1813 target genes domeless and Socs36E. CONCLUSIONS:Conditional overexpression of upd1 in different tissues of Drosophila imago induces pro-aging or pro-longevity effects in tissue-dependent manner. The effects of upd1 overexpression on lifespan are accompanied by the transcription activation of genes for the components of JAK/duanyu1813 pathway.

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