lncRNA-RMRP promotes proliferation, migration and invasion of bladder cancer via miR-206.

OBJECTIVE:The incidence of bladder cancer (BC) is common in the world, but its detail mechanisms for occurrence and development remain unclear. Recently, long non-coding RNAs (lncRNAs) have been observed to play an important role in many different diseases. In this research, we mainly explored the role of the RNA component of mitochondrial RNA processing endoribonuclease in bladder cancer. MATERIALS AND METHODS:We used qRT-PCR to detect the expression of in bladder cancer patients and tumor cells, and the clinical significance was also analyzed. The methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation, and we used transwell to detect the migration and invasion, after the lncRNA was inhibited. Western-blot was used to measure the relative protein expression level in bladder cancer cells after transfection with siRNA-NC or found that the lncRNA duanyu1748P was highly expressed in bladder cancer tissue, compared with adjacent tissue. We also found that the expression of duanyu1748P was closely related with the size, lymph node metastasis and survival time of patients. What's more, duanyu1748P could promote the proliferation, migration and invasion of BC cell lines via regulating miR-206 as a sponge. CONCLUSIONS:According to the results, we found that lncRNA duanyu1748P was closely related to the progression of bladder cancer, which could be a potential target for treating BC patients.

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