Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.

Methods Mol. Biol.2019;1920:183-218. doi:10.1007/978-1-4939-9009-2_12

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Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

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Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections.

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