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CIPK11-Dependent Phosphorylation Modulates FIT Activity to Promote Arabidopsis Iron Acquisition in Response to Calcium Signaling.

Dev Cell. 2019 Mar 11;48(5):726-740.e10. Epub 2019 Jan 31
Regina Gratz 1 , Prabha Manishankar 2 , Rumen Ivanov 1 , Philipp Köster 2 , Inga Mohr 1 , Ksenia Trofimov 1 , Leonie Steinhorst 2 , Johannes Meiser 1 , Hans-Jörg Mai 1 , Maria Drerup 2 , Sibylle Arendt 2 , Michael Holtkamp 3 , Uwe Karst 3 , Jörg Kudla 2 , Petra Bauer 4 , Tzvetina Brumbarova 5
Regina Gratz 1 , Prabha Manishankar 2 , Rumen Ivanov 1 , Philipp Köster 2 , Inga Mohr 1 , Ksenia Trofimov 1 , Leonie Steinhorst 2 , Johannes Meiser 1 , Hans-Jörg Mai 1 , Maria Drerup 2 , Sibylle Arendt 2 , Michael Holtkamp 3 , Uwe Karst 3 , Jörg Kudla 2 , Petra Bauer 4 , Tzvetina Brumbarova 5
+ et al

[No authors listed]

Author information
  • 1 Institute of Botany, Heinrich-Heine University, Düsseldorf 40225, Germany.
  • 2 Institute of Plant Biology and Biotechnology, University of Münster, Münster 48149, Germany.
  • 3 Institute of Inorganic and Analytical Chemistry, University of Münster, Münster 48149, Germany.
  • 4 Institute of Botany, Heinrich-Heine University, Düsseldorf 40225, Germany; Cluster of Excellence on Plant Sciences, Heinrich-Heine University, Düsseldorf 40225, Germany. Electronic address: petra.bauer@uni-duesseldorf.de.
  • 5 Institute of Botany, Heinrich-Heine University, Düsseldorf 40225, Germany. Electronic address: tzvetina.brumbarova@uni-duesseldorf.de.

摘要

Nutrient acquisition is entangled with growth and stress in sessile organisms. The bHLH transcription factor FIT is a key regulator of Arabidopsis iron (Fe) acquisition and post-translationally activated upon low Fe. We identified CBL-INTERACTING PROTEIN KINASE CIPK11 as a FIT interactor. Cytosolic Ca2+ concentration and CIPK11 expression are induced by Fe deficiency. cipk11 mutant plants display compromised root Fe mobilization and seed Fe content. Fe uptake is dependent on CBL1/CBL9. CIPK11 phosphorylates FIT at Ser272, and mutation of this target site modulates FIT nuclear accumulation, homo-dimerization, interaction with bHLH039, and transcriptional activity and affects the plant's Fe-uptake ability. We propose that Ca2+-triggered CBL1/9-mediated activation of CIPK11 and subsequent phosphorylation of FIT shifts inactive into active FIT, allowing regulatory protein interactions in the nucleus. This biochemical link between Fe deficiency and the cellular Ca2+ decoding machinery represents an environment-sensing mechanism to adjust nutrient uptake. Copyright © 2019 Elsevier Inc. All rights reserved.

KEYWORDS: CBL, CIPK11, FIT, bHLH039, iron deficiency, nucleocytoplasmic partitioning, protein interaction, protein mobility, protein phosphorylation, stress

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