DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW.

Curr Biol. 2019 Feb 04;29(3):520-529.e6. Epub 2019 Jan 24

Wouter Smet ¹ , Iris Sevilem ² , Maria Angels de Luis Balaguer ³ , Brecht Wybouw ⁴ , Eliana Mor ⁴ ,

Wouter Smet ¹ , Iris Sevilem ² , Maria Angels de Luis Balaguer ³ , Brecht Wybouw ⁴ , Eliana Mor ⁴ , Shunsuke Miyashima ⁵ , Bernhard Blob ⁶ , Pawel Roszak ⁷ , Thomas B Jacobs ⁴ , Mark Boekschoten ⁸ , Guido Hooiveld ⁸ , Rosangela Sozzani ³ , Ykä Helariutta ⁹ , Bert De Rybel ¹⁰

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¹ Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium; Wageningen University, Laboratory of Biochemistry, Stippeneng 4, 6708 WE Wageningen, the Netherlands.
² Institute of Biotechnology, HiLIFE, University of Helsinki, Viikinkaari 5d, 00014 Helsinki, Finland; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00014 Helsinki, Finland.
³ Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.
⁴ Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium.
⁵ Institute of Biotechnology, HiLIFE, University of Helsinki, Viikinkaari 5d, 00014 Helsinki, Finland; Graduate School of Sciences and Technology, Nara Institute of Science and Technology, Nara, 630-0192 Japan.
⁶ Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.
⁷ Institute of Biotechnology, HiLIFE, University of Helsinki, Viikinkaari 5d, 00014 Helsinki, Finland; Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK.
⁸ Wageningen University, Division of Human Nutrition and Health, Nutrition, Metabolism and Genomics group, Stippeneng 4, 6708 WE Wageningen, the Netherlands.
⁹ Institute of Biotechnology, HiLIFE, University of Helsinki, Viikinkaari 5d, 00014 Helsinki, Finland; Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00014 Helsinki, Finland; Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge CB2 1LR, UK. Electronic address: yrjo.helariutta@slcu.cam.ac.uk.
¹⁰ Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium; VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium; Wageningen University, Laboratory of Biochemistry, Stippeneng 4, 6708 WE Wageningen, the Netherlands. Electronic address: beryb@psb.vib-ugent.be.

摘要

To create a three-dimensional structure, plants rely on oriented cell divisions and cell elongation. Oriented cell divisions are specifically important in procambium cells of the root to establish theÂ different vascular cell types [1, 2]. These divisions are in part controlled by the auxin-controlled TARGET OF (TMO5) and LONESOME HIGHWAY (LHW) transcription factor complex [3-7]. Loss-of-function of tmo5 or lhw clade members results in strongly reduced vascular cell file numbers, whereas ectopic expression of both TMO5 and LHW can ubiquitously induce periclinal and radial cell divisions in all cell types of the root meristem. TMO5 and LHW interact only in young xylem cells, where they promote expression of two direct target genes involved in the final step of cytokinin (CK) biosynthesis, LONELY GUY3 (LOG3) and LOG4 [8, 9] Therefore, CK was hypothesized to act as a mobile signal from the xylem to trigger divisions in the neighboring procambium cells [3, 6]. To unravel how TMO5/LHW-dependent cytokinin regulates cell proliferation, we analyzed the transcriptional responses upon simultaneous induction of both transcription factors. Using inferred network analysis, we identified AT2G28510/DOF2.1 as a cytokinin-dependent downstream target gene. We further showed that DOF2.1 controls specific procambium cell divisions without inducing other cytokinin-dependent effects such as the inhibition of vascular differentiation. In summary, our results suggest that DOF2.1 and its closest homologs control vascular cell proliferation, thus leading to radial expansion of the root.

KEYWORDS: DOF-type transcription factor, cytokinin signaling, plant vascular development, procambium divisions

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