Genetic analysis of the RNA polymerase II CTD in Drosophila.
Methods. 2019 Apr 15;159-160:129-137. Epub 2019 Jan 24
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摘要
The Carboxy-terminal Domain (CTD) of RNA polymerase II (Pol II) plays essential roles in regulating gene expression in eukaryotes. Here, we describe multiple genetic approaches for studying the CTD in Drosophila that complement pre-existing molecular analyses of the Pol II CTD in other experimental models. These approaches will allow one to assess the effects of any CTD mutations in a developmentally complex organism. The approaches discussed in this work can in principle, be applied to analyze other transcription components in eukaryotes.
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基因功能
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原始数据
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文献解读
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