Atypical protein kinase C isoforms differentially regulate directional keratinocyte migration during wound healing.

BACKGROUND:The epidermis possesses regenerative properties that become apparent only after wounding. Atypical protein kinase C isoforms and form a ternary complex with Par3 and Par6, and play crucial roles in establishing and maintaining epithelial cell polarity. The epidermal loss of in progressive depletion of hair follicle stem cells. However, it is unclear whether have equivalent activities in epidermal regeneration. OBJECTIVES:To clarify functional differences between aduanyu1531ζ and aduanyu1531λ in cutaneous wound healing. METHODS:We compared cutaneous wound healing processes in vivo using mutant mice with genetic deletion of each isoform. We also analyzed functional differences between aduanyu1531ζ and aduanyu1531λ in cell proliferation, directional cell migration, and formation of microtubules in vitro using primary keratinocytes established from each mutant mouse. RESULTS:Wound healing was significantly retarded in epidermis-specific aduanyu1531λ knockout mice. In keratinocytes, the correct orientation of cell protrusions toward the wound was disrupted through the destabilization of Par6β. The elongation of stabilized β-tubulin was also deteriorated in aduanyu1531λ-deleted keratinocytes, leading to defects in cell spreading. Conversely, wound healing and directional cell migration in mice were comparable to those in their control are not functionally equivalent; but not plays a primary role in cutaneous wound healing.

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