[No authors listed]
An increasing number of studies have indicated that the dysregulation of microRNAs (miRNAs/miR) is closely associated with nonâsmall cell lung cancer (NSCLC) development and progression by acting as tumor suppressors or oncogenes. Therefore, an inâdepth understanding of the biological roles of miRNAs in NSCLC may provide novel therapeutic methods for the treatment of patients with this disease. In the present study, reverse transcriptionâquantitative polymerase chain reaction was used to detect miRâ577 expression in NSCLC tissues and cell lines. Cell Counting Kitâ8 and Transwell invasion assays were performed to determine the effects of miRâ577 on NSCLC cell proliferation and invasion. Luciferase reporter assays were used to demonstrate the relationship between miRâ577 and homeobox A1 (HOXA1) in NSCLC cells. The results revealed that miRâ577 was markedly downregulated in NSCLC tissues and cell lines. Additionally, restoration of miRâ577 expression significantly decreased the proliferation and invasion of NSCLC cells. Furthermore, miRâ577 negatively regulated HOXA1 expression in NSCLC cells by directly binding to its 3'âuntranslated region. HOXA1 was significantly upregulated in NSCLC tissues, and its upregulation was inversely correlated with miRâ577. Notably, restored HOXA1 expression abrogated the reduced proliferation and invasion of NSCLC cells caused by miRâ577 overexpression. Taken together, these results indicated that miRâ577 may have served tumor suppressive roles in NSCLC by directly targeting HOXA1. Therefore, this miRNA may be developed as a potential therapeutic target for the therapy of patients with NSCLC.
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