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TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network.

Nat Commun. 2018 Dec 14;9(1):5313
Luciana Renna 1 , Giovanni Stefano 2 , Erin Slabaugh 3 , Clarissa Wormsbaecher 4 , Alan Sulpizio 5 , Krzysztof Zienkiewicz 6 , Federica Brandizzi 7
Luciana Renna 1 , Giovanni Stefano 2 , Erin Slabaugh 3 , Clarissa Wormsbaecher 4 , Alan Sulpizio 5 , Krzysztof Zienkiewicz 6 , Federica Brandizzi 7
+ et al

[No authors listed]

Author information
  • 1 MSU-DOE Plant Research Lab, Michigan State University, East Lansing, MI, 48824, USA.
  • 2 Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.
  • 3 Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, NC, 27695, USA.
  • 4 Department of Molecular Genetics, The Ohio State University, Columbus, OH, 43210, USA.
  • 5 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 14850, USA.
  • 6 Department of Plant Biochemistry, Georg-August-University, Albrecht-von-Haller-Institute for Plant Sciences, 37073, Göttingen, Germany.
  • 7 Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA. fb@msu.edu.

摘要


Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, the Trans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.