例如:"lncRNA", "apoptosis", "WRKY"

HOTAIR participates in hepatic insulin resistance via regulating SIRT1.

Eur Rev Med Pharmacol Sci. 2018 Nov;22(22):7883-7890. doi:10.26355/eurrev_201811_16414
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摘要


OBJECTIVE:The aim of this study was to investigate the effect of long non-coding RNA (lncRNA) HOTAIR on hepatic insulin resistance and to explore the possible underlying mechanism. PATIENTS AND METHODS:Liver tissues of type 2 diabetes mellitus (T2D) patients, healthy controls, C57BL/6J mice fed with a high-fat diet and db/db mice were harvested. Meanwhile, the expressions of HOTAIR and SIRT1 were detected. Subsequently, We HepG2 cells were treated with tumor necrosis factor α (TNF-α), and the insulin resistance model was constructed in vitro. The mRNA expression levels of HOTAIR and SIRT1 in the insulin resistance model were detected by quantitative Real (qRT-PCR). Insulin sensitivity in HepG2 cells transfected with lentivirus was evaluated by relative commercial kits. In addition, protein expression levels of key factors in the AKT/GSK pathway were detected by Western blot. RESULTS:HOTAIR was significantly upregulated in T2D patients, C57BL/6J mice fed with a high-fat diet and db/db mice. However, SIRT1 expression presented an opposite changing trend. Moreover, upregulated HOTAIR remarkably promoted hepatic insulin resistance via the AKT/GSK pathway, which could be reversed by SIRT1 overexpression. CONCLUSIONS:Upregulated HOTAIR promotes hepatic insulin resistance by inhibiting SIRT1 expression and AKT/GSK pathway.

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