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RNF135 is a positive regulator of IFN expression and involved in RIG-I signaling pathway by targeting RIG-I.

Fish Shellfish Immunol.2019 Mar;86:474-479. Epub 2018 Nov 30
Yuxiong Lai 1 , Min Liang 2 , La Hu 3 , Zicheng Zeng 4 , Hai Lin 5 , Gao Yi 6 , Ming Li 7 , Zhaoyu Liu 8
Yuxiong Lai 1 , Min Liang 2 , La Hu 3 , Zicheng Zeng 4 , Hai Lin 5 , Gao Yi 6 , Ming Li 7 , Zhaoyu Liu 8
+ et al

[No authors listed]

Author information
  • 1 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: laiyuxiong163@163.com.
  • 2 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: liangmin201325@hotmail.com.
  • 3 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: sysu1924@outlook.com.
  • 4 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: 1121139898@qq.com.
  • 5 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: 308705908@qq.com.
  • 6 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: GYWYer@126.com.
  • 7 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: mingli_1984@gzhmu.edu.cn.
  • 8 Department of Center Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510700, China. Electronic address: gywyer@gzhmu.edu.cn.

摘要

RIG-I-like receptors (RLRs) play a key role in antiviral and inflammatory responses. Increasing evidence indicates that ubiquitination is crucial for regulation of RIG-I signaling pathway. Several ubiquitin ligases were reported to be involved in RIG-I-mediated signal transduction. In the present study, we demonstrated zebrafish RING finger protein 135 (zbRNF135) was a critical player in the regulation of RIG-I signaling pathway. zbRNF135 mRNA was widely expressed in different tissues of zebrafish. The expression of zbRNF135 was up-regulated post poly(I:C) treatment in vivo and in vitro. Furthermore, the expression profiles of RIG-I signaling pathway related genes (LGP2, MDA5, RIG-I, MAVS, TRAF3, IRF3 and IRF7), together with its downstream molecules (IFN1, ISG15, Mx and PKR), were up-regulated by overexpression of zbRNF135 in ZF4 cells. Luciferase and ubiquitination assays revealed that overexpression of zbRNF135 facilitated zebrafish RIG-I (zbRIG-I)-mediated IFN1 promoter activation by mediating K63-linked ubiquitination of zbRIG-I. The co-immunoprecipitation assay showed that zbRNF135 specifically interacted with zbRIG-I. Our study indicated that zbRNF135 participated in innate immune response through modulating RIG-I signaling pathway.

KEYWORDS: IFN, RIG-I, RNF135, Ubiquitination, Zebrafish

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