例如:"lncRNA", "apoptosis", "WRKY"

Crosstalk of oxidative damage, apoptosis, and autophagy under endoplasmic reticulum (ER) stress involved in thifluzamide-induced liver damage in zebrafish (Danio rerio).

Environ. Pollut.2018 Dec;243(Pt B):1904-1911. Epub 2018 Oct 06
Yang Yang 1 , Fengshou Dong 1 , Xingang Liu 1 , Jun Xu 1 , Xiaohu Wu 1 , Wenxian Liu 2 , Yongquan Zheng 3
Yang Yang 1 , Fengshou Dong 1 , Xingang Liu 1 , Jun Xu 1 , Xiaohu Wu 1 , Wenxian Liu 2 , Yongquan Zheng 3
+ et al

[No authors listed]

Author information
  • 1 State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China.
  • 2 State Key Laboratory of Grassland Agro-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou, 730029, PR China.
  • 3 State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, PR China. Electronic address: zhengyongquan@ippcaas.cn.

摘要


Although the hepatotoxicity of thifluzamide in zebrafish has been characterized, its toxic mechanisms have not been fully explored. The present study demonstrated that thifluzamide damaged the zebrafish liver and endoplasmic reticulum (ER). In addition, thifluzamide significantly changed the expression of genes encoding antioxidant proteins and increased the malondialdehyde (MDA) content, leading to oxidative damage in zebrafish liver. Additionally, the autophagic ultrastructure was observed by transmission electron microscopy (TEM), and LC3-I/LC3-II conversion was obviously upregulated under western blotting (WB) measurements, verifying that autophagy was induced by thifluzamide. Moreover, the activities of Caspase-3 and Caspase-9 were obviously decreased, indicating that apoptosis was inhibited in adult zebrafish exposed to a higher concentration of thifluzamide. In summary, oxidative damage and autophagy but not apoptosis under ER injury might lead to the hepatotoxicity of thifluzamide in zebrafish. Our findings provide a new mechanistic insight into the toxicity of thifluzamide in zebrafish.

KEYWORDS: Autophagy, ER, Oxidative damage, Thifluzamide, Zebrafish