Profilin facilitates PKCε activation by accelerating ATP supply.

Profilin catalyzes the exchange of actin-bound ADP to ATP. The present study investigated the role of profilin in activation. Profilin associated with duanyu1531ε in differentiated PC-12 cells under the basal conditions, which was inhibited by the inhibitor GF109203X. The selective duanyu1531ε activator DCP-LA markedly increased the association, which was clearly prevented by GF109203X. The basal duanyu1531 activity in PC-12 cells was attenuated by knocking-down profilin, while the basal activities of and CaMKII were not affected. DCP-LA enhanced the duanyu1531 activity to approximately 3.5 folds of the basal levels, and the effect was suppressed by knocking-down profilin. In the cell-free system, duanyu1531ε was not activated by profilin alone. DCP-LA activated duanyu1531ε in an ATP concentration (2-500 μM)-dependent manner, and addition of profilin shifted the ATP concentration/DCP-LA-induced duanyu1531ε activity relation curve to the left (the direction of lower ATP concentrations). Taken together, the results of the present study indicate that profilin binds to activated duanyu1531ε and facilitates duanyu1531ε activation by accelerating ATP supply to

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