Exposure to triclosan changes the expression of microRNA in male juvenile zebrafish (Danio rerio).

Triclosan (TCS) is a broad-spectrum antibacterial agent which is widely used in various personal care products and cosmetics. It has been found that TCS affects endocrine, immune, nervous, reproductive, and developmental system. Although microRNAs (miRNAs) act a pivotal part in lots of metabolic activities, whether and how they are related to the process of TCS-induced toxicity is unknown. In the present study, TCS induced changes in miRNAs and target gene expression in male zebrafish (Danio rerio) brain, and the potential mechanism was studied. Male juvenile zebrafish were exposed to 0 and 68 μg/L TCS for 42 d. miRNA was isolated from the brain pool of the zebrafish and the expression profiles of 255 known zebrafish miRNAs were analysed by using Affymetrix miRNA 4.0 microarrays. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assay the expression of 5 differentially expressed miRNAs in the microarray data and some related-genes in brains. The GO term analysis revealed that miRNAs significantly affected by TCS exposure were mainly involved in translation, transcription, DNA-templated, protein transport, and motor neuron axon guidance biological process. Pathway analysis showed that target genes of 5 differentially expressed miRNAs prominently participate in basal transcription factors, purine metabolism, and ribosome biogenesis in eukaryotes. In addition, key genes in purine metabolism pathway and oxidative stress related-genes were significantly changed. These findings offer novel insight into the mechanisms of epigenetic regulation in TCS-induced toxicity in male zebrafish, and distinguish novel miRNA biomarkers for exposure to TCS.

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