例如:"lncRNA", "apoptosis", "WRKY"

MiR-940 inhibits the progression of NSCLC by targeting FAM83F.

Eur Rev Med Pharmacol Sci. 2018 Sep;22(18):5964-5971. doi:10.26355/eurrev_201809_15927
{{ author.authorName }}{{getOrganisationIndexOf(author)}} {{ author.authorName }}{{getOrganisationIndexOf(author)}}
{{ author.authorName }}{{getOrganisationIndexOf(author)}} {{ author.authorName }}{{getOrganisationIndexOf(author)}}
+ et al

[No authors listed]

Author information
  • {{index+1}} {{ organisation }}

摘要


OBJECTIVE:We investigate whether miR-940 could target family sequence similarity 83 member F (FAM83F) and further inhibit the progression of non-small cell lung cancer (NSCLC). PATIENTS AND METHODS:The expression levels of miR-940 and FAM83F in tumor tissues and paracancerous tissues of 72 NSCLC patients were detected through quantitative real time-polymerase chain reaction (qRT-PCR). The relationship between their expression levels, tumor size, and prognosis of NSCLC was analyzed. Transfection plasmids were constructed to knockdown or overexpress miR-940 in H1299 cells (inhibitor group) and SK-MES-1 cells (mimic group). The viability of H1299 cells and SK-MES-1 cells was evaluated using cell counting kit-8 (CCK-8) assay after transfection. The combination of miR-940 and Ago2 was confirmed by RNA immunoprecipitation (RIP) experiment. The binding condition of miR-940 in FAM83F-WT and FAM83F-MUT groups was verified by luciferase reporter gene assay. RESULTS:MiR-940 expression was noticeably decreased, while FAM83F expression was distinctly upregulated in NSCLC tissues than that of paracancerous tissues. The overall survival rate of NSCLC patients with highly-expressed miR-940 was significantly higher than those with lowly-expressed miR-940. Besides, miR-940 level was negatively correlated with tumor stage and size of NSCLC patients. Knockdown of miR-940 evidently enhanced the activity of H1299 cells, while overexpression of miR-940 decreased the viability of SK-MES-1 cells. In addition, miR-940 was confirmed to combine with FAM83F. Luciferase activity of cells co-transfected with FAM83F-WT and miR-940 mimic was significantly decreased. CONCLUSIONS:MiR-940 inhibited the proliferation of cancer cells by targeting FAM83F and further restrained the progression of NSCLC.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}

基因功能


  • {{$index+1}}.{{ gene }}

图表


原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
{{attr}}
{{ dataList.sampleTitle }}
{{ dataList.organism }} {{ dataList.expermentTitle }} {{ dataList.sampleType }} {{ dataList.libraryInstrument }} {{ showAttributeName(index,attr,dataList.attributes) }}

文献解读