[No authors listed]
Vascular endothelial growth factor (VEGF) promotes angiogenesis during tumor growth, and its expression involves multiple signaling pathways and transcription factors. In the present study, transforming growth factor (TGF)âβ1 promoted upregulation of VEGF and downregulation of microRNA (miR)â20b expression in mouse H22 hepatocellular carcinoma cells. miRâ20b negatively regulated both constitutive VEGF expression and TGFâβ1âinduced VEGF expression. The miRanda algorithm predicted that a binding site of the miRâ20b GCAAUCUGGGCACUUU sequence was present in the signal transducer and activator of transcription 3'âuntranslated region. Following transfection of miRâ20b mimics into H22 cells, expression of protein was downregulated. A dualâluciferase activity assay revealed that miRâ20b directly targeted duanyu18133 to regulate its expression, and that interference with duanyu18133 expression significantly downregulated VEGF mRNA and protein expression. Interference with duanyu18133 expression resulted in increased VEGF expression in H22 cells overexpressing miRâ20b, but expression was lower than that in quiescent H22 cells. This indicated that duanyu18133 was involved in the negative regulation of VEGF expression in H22 cells by miRâ20b. The data demonstrated that miRâ20b negatively regulated VEGF expression by directly targeting duanyu18133 in H22 cells.
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