例如："lncRNA", "apoptosis", "WRKY"

MicroRNA-224 down-regulates Glycine N-methyltransferase gene expression in Hepatocellular Carcinoma.

Sci Rep. 2018 Aug 16;8(1):12284
Jung-Hsien Hung 1 , Chung-Hsien Li 1 , Ching-Hua Yeh 1 , Pin-Cheng Huang 1 , Cheng-Chieh Fang 1 , Yen-Fu Chen 1 , Kuo-Jui Lee 1 , Chih-Hung Chou 2 , Hsin-Yun Cheng 1 , Hsien-Da Huang 3 , Marcelo Chen 4 , Ting-Fen Tsai 5 , Anya Maan-Yuh Lin 6 , Chia-Hung Yen 7 , Ann-Ping Tsou 8 , Yu-Chang Tyan 1 , Yi-Ming Arthur Chen 9
Jung-Hsien Hung 1 , Chung-Hsien Li 1 , Ching-Hua Yeh 1 , Pin-Cheng Huang 1 , Cheng-Chieh Fang 1 , Yen-Fu Chen 1 , Kuo-Jui Lee 1 , Chih-Hung Chou 2 , Hsin-Yun Cheng 1 , Hsien-Da Huang 3 , Marcelo Chen 4 , Ting-Fen Tsai 5 , Anya Maan-Yuh Lin 6 , Chia-Hung Yen 7 , Ann-Ping Tsou 8 , Yu-Chang Tyan 1 , Yi-Ming Arthur Chen 9
+ et al

[No authors listed]

Author information
  • 1 Center for Infectious Disease and Cancer Research (CICAR), Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 2 Institute of Bioinformatics and Systems Biology, National Chiao Tung University, HsinChu, Taiwan.
  • 3 Department of Biological Science and Technology, National Chiao Tung University, HsinChu, Taiwan.
  • 4 Department of Cosmetic Applications and Management, Mackay Junior College of Medicine, Nursing and Management, Taipei, Taiwan.
  • 5 Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.
  • 6 Faculty of Pharmacy, Taipei, Taiwan, National Yang-Ming University, Taipei, Taiwan.
  • 7 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
  • 8 Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
  • 9 Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan. arthur@kmu.edu.tw.

摘要

Glycine N-methyltransferase (GNMT) is a tumor suppressor for HCC. It is down-regulated in HCC, but the mechanism is not fully understood. MicroRNA-224 (miR-224) acts as an onco-miR in HCC. This study is the first to investigate miR-224 targeting the coding region of GNMT transcript. The GNMT-MT plasmid containing a miR-224 binding site silent mutation of the GNMT coding sequence can escape the suppression of miR-224 in HEK293T cells. Expression of both exogenous and endogenous GNMT was suppressed by miR-224, while miR-224 inhibitor enhanced GNMT expression. miR-224 counteracts the effects of GNMT on the reduction of cell proliferation and tumor growth. The levels of miR-224 and GNMT mRNA showed a significant inverse relationship in tumor specimens from HCC patients. Utilizing CCl4-treated hepatoma cells and mice as a cell damage of inflammatory or liver injury model, we observed that the decreased expression levels of GNMT were accompanied with the elevated expression levels of miR-224 in hepatoma cells and mouse liver. Finally, hepatic AAV-mediated GNMT also reduced CCl4-induced miR-224 expression and liver fibrosis. These results indicated that AAV-mediated GNMT has potential liver protection activity. miR-224 can target the GNMT mRNA coding sequence and plays an important role in GNMT suppression during liver tumorigenesis.

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