例如:"lncRNA", "apoptosis", "WRKY"

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell Rep. 2018 Aug 14;24(7):1756-1764
Inna Goliand 1 , Shai Adar-Levor 1 , Inbar Segal 1 , Dikla Nachmias 1 , Tali Dadosh 2 , Michael M Kozlov 3 , Natalie Elia 4
Inna Goliand 1 , Shai Adar-Levor 1 , Inbar Segal 1 , Dikla Nachmias 1 , Tali Dadosh 2 , Michael M Kozlov 3 , Natalie Elia 4
+ et al

[No authors listed]

Author information
  • 1 Department of Life Sciences and NIBN, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel.
  • 2 Department of Chemical Research Support, Faculty of Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.
  • 3 Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
  • 4 Department of Life Sciences and NIBN, Ben-Gurion University of the Negev, Beer Sheva 84105, Israel. Electronic address: elianat@post.bgu.ac.il.

摘要


The ESCRT machinery mediates membrane fission in a variety of processes in cells. According to current models, ESCRT-III proteins drive membrane fission by assembling into helical filaments on membranes. Here, we used 3D STORM imaging of endogenous ESCRT-III component IST1 to reveal the evolution of the structural organization of ESCRT-III in mammalian cytokinetic abscission. Using this approach, ESCRT-III ring and spiral assemblies were resolved and characterized at different stages of abscission. Visualization of IST1 structures in cells lacking the microtubule-severing enzyme spastin and in cells depleted of specific ESCRT-III components or the ATPase VPS4 demonstrated the contribution of these components to the organization and function of ESCRTs in cells. This work provides direct evidence that ESCRT-III proteins form helical filaments to mediate their function in cells and raises new mechanistic scenarios for ESCRT-driven cytokinetic abscission.

KEYWORDS: ESCRT machinery, abscission, cytokinesis, membrane fission, super resolution microscopy