例如:"lncRNA", "apoptosis", "WRKY"

A nuclear-encoded protein, mTERF6, mediates transcription termination of rpoA polycistron for plastid-encoded RNA polymerase-dependent chloroplast gene expression and chloroplast development.

Sci Rep. 2018 Aug 09;8(1):11929
Yi Zhang 1 , Yong-Lan Cui 2 , Xiao-Lei Zhang 2 , Qing-Bo Yu 2 , Xi Wang 2 , Xin-Bo Yuan 2 , Xue-Mei Qin 2 , Xiao-Fang He 2 , Chao Huang 2 , Zhong-Nan Yang 3
Yi Zhang 1 , Yong-Lan Cui 2 , Xiao-Lei Zhang 2 , Qing-Bo Yu 2 , Xi Wang 2 , Xin-Bo Yuan 2 , Xue-Mei Qin 2 , Xiao-Fang He 2 , Chao Huang 2 , Zhong-Nan Yang 3
+ et al

[No authors listed]

Author information
  • 1 Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, Shanghai, 201602, China.
  • 2 College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China.
  • 3 College of Life and Environmental Sciences, Shanghai Normal University, Shanghai, 200234, China. znyang@shnu.edu.cn.

摘要


The expression of plastid genes is regulated by two types of DNA-dependent RNA polymerases, plastid-encoded RNA polymerase (PEP) and nuclear-encoded RNA polymerase (NEP). The plastid rpoA polycistron encodes a series of essential chloroplast ribosome subunits and a core subunit of PEP. Despite the functional importance, little is known about the regulation of rpoA polycistron. In this work, we show that mTERF6 directly associates with a 3'-end sequence of rpoA polycistron in vitro and in vivo, and that absence of mTERF6 promotes read-through transcription at this site, indicating that mTERF6 acts as a factor required for termination of plastid genes' transcription in vivo. In addition, the transcriptions of some essential ribosome subunits encoded by rpoA polycistron and PEP-dependent plastid genes are reduced in the mterf6 knockout mutant. RpoA, a PEP core subunit, accumulates to about 50% that of the wild type in the mutant, where early chloroplast development is impaired. Overall, our functional analyses of mTERF6 provide evidence that it is more likely a factor required for transcription termination of rpoA polycistron, which is essential for chloroplast gene expression and chloroplast development.