Gene Expression of Glyoxalase II in Diabetic Retinopathy.

OBJECTIVE:To study the gene expression of Glyoxalase II among patients of diabetic retinopathy. STUDY DESIGN:A cross-sectional comparative study. PLACE AND DURATION OF STUDY:CREAM (Centre for Research in Experimental and Applied Medicine) and the Department of Biochemistry and Army Medical College, Rawalpindi in collaboration with Armed Forces Institute of Ophthalmology (AFIO), Rawalpindi, from November 2015 to November 2016. METHODOLOGY:Individuals were enrolled, among whom 30 were cases with diabetic retinopathy and 30 were controls without the disease. Their relevant data were collected and blood samples were drawn. Individual RNA was extracted from respective samples and cDNA was synthesised from each. Expression analysis for Glyoxalase II was done and relative quantification was done using delta delta CT method. RESULTS:A total of 60 individuals of ages 40-70 years were enrolled in the study, 30 cases and 30 controls. Among these, 34 (56.67%) were males and 26 (43.3%) were females. Mean ages were 60 ±8 years in cases and 59 ±13 years in controls. Down regulation of Glyoxalase II was observed in cases as compared to controls. CONCLUSION:Downregulation of Glyoxalase II, seen among patients of diabetic retinopathy, may indicate a failure of detoxifying system leading to accumulation of glycated end products.

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