[No authors listed]
Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF-P) is required for the stalled ribosome to continue translation and thus the absence of EF-P affects translation of the associated open reading frame. Here we report that EF-P controls translation of the mgtA gene encoding a Mg2+ -transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF-P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF-P-mediated control of MgtA protein without affecting the Mg2+ -transporting activity of the mgtA gene. The Pro550 and Pro551-substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF-P-mediated translational control of the mgtA gene is required for Salmonella pathogenesis.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |