Regulation of the regulator of G protein signaling 2 expression and cellular localization by PKA and PKC pathways in mouse granulosa cells.

G protein-coupled receptor (GPCR) activation-mediated and pathways have been recognized to be important in ovarian physiology. Expression of regulator of G-protein signaling 2 (RGS2) has been reported in ovarian granulosa cells. The detailed mechanisms in and RGS2 expression and cellular translocation in granulosa cells remain mostly unclear. duanyu1529 activator 8-bromo-cAMP and duanyu1531 activator phorbol-12, 13-didecanoate appeared to rapidly elevate both protein and mRNA levels and promoter activation of RGS2 gene. Two consensus Sp1 elements within the shortest 78 bp fragment of RGS2 promoter sequence were essential for the full responsiveness to duanyu1529 and duanyu1531 activation appeared to increase the RGS2 translocation from nucleus to cytosol. duanyu1529- and RGS2 transcription in a Sp-1-dependent manner and a duanyu1531-mediated RGS2 intracellular translocation were noted in granulosa cells.

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