Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.

Nucleic Acids Res.1985 Mar 25;13(6):2063-74. doi:10.1093/nar/13.6.2063

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The aspA gene of Escherichia coli W which encodes aspartase was cloned into the plasmid vector pBR322. The nucleotide sequences of aspA and its flanking regions were determined. The aspA gene encodes a protein with a molecular weight of 52,224 consisted of 477 amino acid residues. The amino acid sequence of the protein predicted from the nucleotide sequence was consistent with those of the NH2- and COOH-terminal regions and also with the amino acid composition of the purified aspartase determined previously. Potential promoter and terminator sequences for aspA were also found in the determined sequence.

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Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W.

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