Pellino-1 Protects Periodontal Ligament Stem Cells Against H₂O₂-Induced Apoptosis via Activation of NF-κB Signaling.

To determine the protective effects of Pellino-1 against H₂O₂-induced apoptosis in periodontal ligament stem cells (PDLSC). We demonstrated that H₂O₂ decreases PDLSC viability by 40 and 50% with the concentrations of 400 and 500 μM, respectively, with an observed downregulation of Pellino-1 mRNA and protein; we further concluded that overexpression of Pellino-1 significantly lowers 8-hydroxy-2'-deoxyguanosine levels by 10% and upregulates superoxide dismutase 1, glutathione peroxidase levels, and catalase mRNA levels by 200, 40, and 250%, respectively. More importantly, we found that overexpression of Pellino-1 inhibited H₂O₂-induced cellular apoptosis through the activation of the NF-κB signaling pathway. Pellino-1 may be critically important for cell survival in the presence of oxidative elements; activation of the NF-κB signaling cascade was required for the overexpression of Pellino-1 to protect the cells from H₂O₂-induced apoptosis.

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