Global transcriptional regulation by BirA in enterohemorrhagic Escherichia coli O157:H7.
[No authors listed]
摘要
AIM:Determination of the effects of BirA on transcription and virulence in enterohemorrhagic Escherichia coli (EHEC) O157:H7. MATERIALS & METHODS:The effect of BirA on EHEC O157:H7 gene expression and phenotypes was assessed by RNA-seq combined with adherence, quantitative biofilm and survival assays. RESULTS:Many genes associated with virulence, amino acid synthesis and transport, and zinc transport were upregulated, whereas genes encoding stress proteins were downregulated in ÎbirA::km+Ac_birA. Accordingly, ÎbirA::km+Ac_birA adhesion to Caco-2 cells, biofilm formation and survival during oxidative stress were higher, whereas its survival during heat shock was lower than that of the wild-type. CONCLUSION:This study demonstrates the wide-ranging regulatory functions of BirA, especially its role in controlling virulence and stress responses in EHEC O157:H7. [Formula: see text].
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基因功能
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原始数据
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文献解读
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