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Suppression of respiratory growth defect of mutant deficient in mitochondrial phospholipase A1 by overexpression of genes involved in coenzyme Q synthesis in Saccharomyces cerevisiae.

Biosci. Biotechnol. Biochem.2018 Sep;82(9):1633-1639. doi:10.1080/09168451.2018.1476124. Epub 2018 May 28
Shiho Morisada 1 , Ikuhisa Nishida 2 , Makoto Kawamukai 2 , Hiroyuki Horiuchi 1 , Ryouichi Fukuda 1
Shiho Morisada 1 , Ikuhisa Nishida 2 , Makoto Kawamukai 2 , Hiroyuki Horiuchi 1 , Ryouichi Fukuda 1

[No authors listed]

Author information
  • 1 a Department of Biotechnology , The University of Tokyo , Tokyo , Japan.
  • 2 b Faculty of Life and Environmental Science, Department of Life Sciences , Shimane University , Matsue , Japan.

摘要


DDL1 encodes a mitochondrial phospholipase A1 involved in acyl chain remodeling of mitochondrial phospholipids and degradation of cardiolipin in Saccharomyces cerevisiae. The deletion of DDL1 leads to respiratory growth defects. To elucidate the physiological role of DDL1, we screened for genes that, when overexpressed, suppress the respiratory growth defect of the DDL1 deletion mutant. Introduction of COQ8, COQ9, or COQ5, which are involved in coenzyme Q (CoQ) synthesis, using a multicopy vector suppressed the respiratory growth defect of the DDL1 deletion mutant. In contrast, introduction of COQ8 using a multicopy vector did not accelerate the growth of the deletion mutants of TAZ1 or CLD1, which encode an acyltransferase or phospholipase A2, respectively, involved in the remodeling of cardiolipin. These results suggest genetic interactions between the mitochondrial phospholipase A1 gene and the genes involved in CoQ synthesis.

KEYWORDS: Saccharomyces cerevisiae, coenzyme Q, mitochondria, phospholipase A1, respiration