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MK2 mediates macrophage activation and acute lung injury by regulating let-7e miRNA.

Am J Physiol Lung Cell Mol Physiol. 2018 Sep 01;315(3):L371-L381. doi:10.1152/ajplung.00019.2018. Epub 2018 May 17
Yaxian Wu 1 , Huiqiong He 1 , Yunhe Ding 1 , Sirui Liu 1 , Depeng Zhang 1 , Jun Wang 1 , Hongchao Jiang 1 , Dan Zhang 2 , Lei Sun 1 , Richard D Ye 3 , Feng Qian 4
Yaxian Wu 1 , Huiqiong He 1 , Yunhe Ding 1 , Sirui Liu 1 , Depeng Zhang 1 , Jun Wang 1 , Hongchao Jiang 1 , Dan Zhang 2 , Lei Sun 1 , Richard D Ye 3 , Feng Qian 4
+ et al

[No authors listed]

Author information
  • 1 Engineering Research Center of Cell and Therapeutic Antibody, Ministry of Education, School of Pharmacy, Shanghai Jiao Tong University , Shanghai , People's Republic of China.
  • 2 Research Center for Cancer Precision Medicine, Department of Medical Oncology, Bengbu Medical College, Bengbu, Anhui , People's Republic of China.
  • 3 Institute of Chinese Medical Sciences, University of Macau, Macau, People's Republic of China.
  • 4 Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University , Xuzhou , People's Republic of China.

摘要


MAPK-activated protein kinase 2 (MK2) plays a critical role in the development of inflammation. However, the modulatory mechanisms in macrophage activation and acute lung injury (ALI) have not been completely defined. Here, we reported that MK2-deficient mice (MK2-/-) protected against sepsis-induced ALI. In response to lipopolysaccharide (LPS) challenge, MK2-/- mice and myeloid cell-specific MK2 conditional knockout mice (MK2Lyz2-KO) exhibited attenuated inflammatory response, especially producing fewer amounts of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, and macrophage inflammatory protein 2 (MIP-2). LPS treatment in vitro resulted in reduced cytokine expression in MK2-/- bone marrow-derived macrophages (BMDMs). Furthermore, we found that LPS-induced microRNA lethal-7e ( let-7e) expression was significantly increased in MK2-/- macrophages. Transfection of let-7e antagomirs into MK2-/- BMDM rescued LPS-induced expression of TNF-α, IL-6, and MIP-2. In contrast, transfection of let-7e mimics into MK2+/+BMDM decreased cytokine expression. Meanwhile, LPS-induced phosphorylation of cAMP response element-binding (CREB) protein, a substrate of MK2, was downregulated in MK2-/- BMDMs. Lin28, an inhibitory molecule of let-7, was significantly reduced in MK2-/- macrophages. Our results suggested that MK2 boosts LPS-induced macrophage activation and ALI via increasing activation of CREB and consequently, the expression of Lin28 and downregulation of let-7e.

KEYWORDS: MAPK-activated protein kinase 2, MK2, acute lung injury, let-7e, lipopolysaccharides, macrophage, miRNA