例如:"lncRNA", "apoptosis", "WRKY"

An In Vivo Screen Identifies PYGO2 as a Driver for Metastatic Prostate Cancer.

Cancer Res.2018 Jul 15;78(14):3823-3833. Epub 2018 May 16
Xin Lu 1 , Xiaolu Pan 2 , Chang-Jiun Wu 3 , Di Zhao 2 , Shan Feng 4 , Yong Zang 5 , Rumi Lee 2 , Sunada Khadka 2 , Samirkumar B Amin 3 , Eun-Jung Jin 6 , Xiaoying Shang 2 , Pingna Deng 2 , Yanting Luo 4 , William R Morgenlander 4 , Jacqueline Weinrich 4 , Xuemin Lu 4 , Shan Jiang 7 , Qing Chang 7 , Nora M Navone 8 , Patricia Troncoso 9 , Ronald A DePinho 10 , Y Alan Wang 10
Xin Lu 1 , Xiaolu Pan 2 , Chang-Jiun Wu 3 , Di Zhao 2 , Shan Feng 4 , Yong Zang 5 , Rumi Lee 2 , Sunada Khadka 2 , Samirkumar B Amin 3 , Eun-Jung Jin 6 , Xiaoying Shang 2 , Pingna Deng 2 , Yanting Luo 4 , William R Morgenlander 4 , Jacqueline Weinrich 4 , Xuemin Lu 4 , Shan Jiang 7 , Qing Chang 7 , Nora M Navone 8 , Patricia Troncoso 9 , Ronald A DePinho 10 , Y Alan Wang 10
+ et al

[No authors listed]

Author information
  • 1 Tumor Microenvironment and Metastasis Program, Indiana University Melvin and Bren Simon Cancer Center, Indianapolis, Indiana.
  • 2 Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • 3 Department of Genomic Medicine, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • 4 Department of Biological Sciences, Center for Rare and Neglected Diseases, University of Notre Dame, Notre Dame, Indiana.
  • 5 Department of Biostatistics, Indiana University, Indianapolis, Indiana.
  • 6 Department of Biological Science, Wonkwang University, Cheonbuk, Iksan, South Korea.
  • 7 Institute for Applied Cancer Science, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • 8 Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • 9 Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas.
  • 10 Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, Texas. rdepinho@mdanderson.org yalanwang@mdanderson.org.

摘要


Advanced prostate cancer displays conspicuous chromosomal instability and rampant copy number aberrations, yet the identity of functional drivers resident in many amplicons remain elusive. Here, we implemented a functional genomics approach to identify new oncogenes involved in prostate cancer progression. Through integrated analyses of focal amplicons in large prostate cancer genomic and transcriptomic datasets as well as genes upregulated in metastasis, 276 putative oncogenes were enlisted into an in vivo gain-of-function tumorigenesis screen. Among the top positive hits, we conducted an in-depth functional analysis on Pygopus family PHD finger 2 (PYGO2), located in the amplicon at 1q21.3. PYGO2 overexpression enhances primary tumor growth and local invasion to draining lymph nodes. Conversely, PYGO2 depletion inhibits prostate cancer cell invasion in vitro and progression of primary tumor and metastasis in vivo In clinical samples, PYGO2 upregulation associated with higher Gleason score and metastasis to lymph nodes and bone. Silencing PYGO2 expression in patient-derived xenograft models impairs tumor progression. Finally, PYGO2 is necessary to enhance the transcriptional activation in response to ligand-induced Wnt/β-catenin signaling. Together, our results indicate that PYGO2 functions as a driver oncogene in the 1q21.3 amplicon and may serve as a potential prognostic biomarker and therapeutic target for metastatic prostate cancer.Significance: Amplification/overexpression of PYGO2 may serve as a biomarker for prostate cancer progression and metastasis. Cancer Res; 78(14); 3823-33. ©2018 AACR.