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[Growth Regulation of Factor Inhibiting Hypoxia-Inducible Factor in Renal Carcinoma Cells].

Sichuan Da Xue Xue Bao Yi Xue Ban. 2018 Jan;49(1):29-33
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摘要


OBJECTIVE:To detect the expression and growth regulation effects of the factor inhibiting hypoxia-inducible factor (FIH-1) in renal carcinoma cells. METHODS:The expressions of FIH-1,hypoxia inducible factor (HIF) and vascular endothelial growth factor (VEGF) were examined in OS-RC-2 and 786-0 cell lines via real-time PCR (RT-PCR) and Western blot. Overexpression plasmid of FIH-1 were designed and constructed as EX-V1104-M45-FIH-1. After the transfection of the plasmid into OS-RC-2 and 786-0 cells,the expression levels of FIH-1,HIF and VEGF were measured by RT-PCR and Western blot,while cell proliferation was tested by MTT. RESULTS:The expressions of FIH-1,HIF-1α,HIF-2α and VEGF were detected in OS-RC-2 cells,and FIH-1,HIF-2α and VEGF were detected in 786-0 cells. After the transfection of EX-V1104-M45-FIH-1, the level of FIH-1 was increased in OS-RC-2,while the levels of HIF-2α,HIF-1α and VEGF were decreased. MTT showed that FIH-1 plasmid transfection decreased the cell proliferation of OS-RC-2,but did not show inhibition on 786-0 cells proliferation. CONCLUSION:FIH-1/HIF-1α/VEGF signaling pathway exists in renal carcinoma cell lines.

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