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Adding a purple corn extract in rats supplemented with chia oil decreases gene expression of SREBP-1c and retains Δ5 and Δ6 hepatic desaturase activity, unmodified the hepatic lipid profile.

Prostaglandins Leukot. Essent. Fatty Acids. 2018 May;132:1-7. Epub 2018 Mar 28
Sixto Reyna Gallegos 1 , Génesis Torres Arrunátegui 2 , Rodrigo Valenzuela 3 , Miguel Ángel Rincón-Cervera 4 , María Elena Villanueva Espinoza 2
Sixto Reyna Gallegos 1 , Génesis Torres Arrunátegui 2 , Rodrigo Valenzuela 3 , Miguel Ángel Rincón-Cervera 4 , María Elena Villanueva Espinoza 2

[No authors listed]

Author information
  • 1 Nutrition Department, Animal Husbandry Faculty, Universidad Nacional Agraria La Molina, Lima, Perú; Carrera de Medicina Veterinaria, Facultad de Ciencias Veterinarias, Universidad Técnica de Manabí, Manabí, Ecuador. Electronic address: sreyna@utm.edu.ec.
  • 2 Nutrition Department, Animal Husbandry Faculty, Universidad Nacional Agraria La Molina, Lima, Perú.
  • 3 Nutrition Department, Faculty of Medicine, Universidad de Chile, Santiago, Chile; Lipid Center, Institute of Nutrition and Food Technology (INTA), University of Chile, Santiago, Chile.
  • 4 Nutrition Department, Faculty of Medicine, Universidad de Chile, Santiago, Chile.

摘要


Flavonoids upregulate gene expression of PPAR-α and underregulate the gene expression of SREBP-1c, and their intake increases the plasmatic concentration of n-3 LC-PUFAs. However, the biological mechanisms underlying these effects have not been elucidated. In this work, the effect of oral supplementation of ALA from chia (Salvia hispanica L.) seed oil and anthocyanins from a purple corn extract (PCE) on gene expression of SREBP-1c, PPAR-α and Δ5 and Δ6 desaturases (Δ5D and Δ6D), the activity of these enzymes in the liver as well as the hepatic lipid profile were evaluated in thirty-six female Sprague Dawley rats whose diet was supplemented with olive oil (OL), chia oil (CH), olive oil and PCE (OL + PCE) or chia oil and PCE (CH + PCE). Gene expression of PPAR-α was significantly higher when supplemented with CH and CH + PCE, SREBP-1c gene expression was higher when supplemented with chia oil. CH supplementation enhanced Δ5D expression whereas no significant differences between treatments were observed concerning Δ6D gene expression. Activities of both desaturases were increased by including olive oil (OL + PCE and OL), and they were found to be higher in CH + PCE respect to CH for both enzymes. The ALA and n-3 LCPUFAs hepatic content was higher with CH, decreasing the levels of AA and n-6 LCPUFAs. It is concluded that the joint action of flavonoids such as anthocyanins and ALA show an anti-adipogenic effect. Desaturase activity was inhibited by ALA and kept by the anthocyanins from PCE, thus anthocyanins would exert a protective effect on the desaturase activity but they would not affect on its gene expression, however, high doses of ALA increased the production of its metabolites, masking the effect of PCE.

KEYWORDS: Chia oil, PPAR, Purple corn, SREBP, Δ5/Δ6 desaturase activity