[No authors listed]
Myocardial ischemiaâreperfusion (I/R) injury is a major cause of cardiovascular disease worldwide, and microRNAs have been implicated in the regulation of pathological and physiological processes in myocardial I/R injury. The present study aimed to investigate the role of microRNA (miR)â221â3p in myocardial I/R injury. Cell death and lactate dehydrogenase (LDH) activity were increased in hydrogen peroxide (H2O2)âtreated H9c2 cells, as measured by flow cytometry and an LDH detection kit. The expression of miRâ221â3p was elevated in H2O2âincubated cells and in remote areas of the rat I/R model, examined using reverse transcriptionâquantitative polymerase chain reaction analysis. The overexpression of miRâ221â3p enhanced the number of propidium iodide (PI)+ cells and the activity of LDH in H2O2âtreated cells. In I/Râinduced rats, the overexpression of miRâ221â3p promoted the number of myosin+ cells and inhibited the fractional shortening of left ventricular diameter (FSLVD%). The results showed that the expression of p57 at the gene and protein levels was decreased in H9c2 cells incubated with H2O2 and in rats subjected to I/R surgery; the expression of p57 decreased following the overexpression of miRâ221â3p. Subsequently, the hypothesis that p57 was the direct target of miRâ221â3p was confirmed by performing a dualâluciferase reporter assay. Finally, to examine the function of p57 in myocardial impairment, p57 was transfected into H9c2 cells and administered to the rats prior to undergoing H2O2 treatment and I/R surgery, respectively. The results indicated that p57 attenuated the number of PI+ cells and the activity of LDH in H2O2âtreated cells, whereas p57 downregulated the number of myosin+ cells and upregulated FSLVD% in the I/Râtreated rats. Therefore, these findings suggested that miRâ221â3p exacerbated the H2O2âinduced myocardial damage in H9c2 cells and myocardial I/R injury in the rat model by modulating p57.
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