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Terminal Uridylyltransferases Execute Programmed Clearance of Maternal Transcriptome in Vertebrate Embryos.

Mol. Cell. 2018 Apr 05;70(1):72-82.e7
Hyeshik Chang 1 , Jinah Yeo 1 , Jeong-Gyun Kim 2 , Hyunjoon Kim 1 , Jaechul Lim 3 , Mihye Lee 1 , Hyun Ho Kim 2 , Jiyeon Ohk 4 , Hee-Yeon Jeon 5 , Hyunsook Lee 5 , Hosung Jung 4 , Kyu-Won Kim 2 , V Narry Kim 6
Hyeshik Chang 1 , Jinah Yeo 1 , Jeong-Gyun Kim 2 , Hyunjoon Kim 1 , Jaechul Lim 3 , Mihye Lee 1 , Hyun Ho Kim 2 , Jiyeon Ohk 4 , Hee-Yeon Jeon 5 , Hyunsook Lee 5 , Hosung Jung 4 , Kyu-Won Kim 2 , V Narry Kim 6
+ et al

[No authors listed]

Author information
  • 1 Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea.
  • 2 Department of Molecular Medicine and Biopharmaceutical Science, Seoul National University, Seoul 08826, Korea.
  • 3 Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
  • 4 Department of Anatomy, Yonsei University College of Medicine, Seoul 03722, Korea.
  • 5 School of Biological Sciences, Seoul National University, Seoul 08826, Korea.
  • 6 Center for RNA Research, Institute for Basic Science, Seoul 08826, Korea; School of Biological Sciences, Seoul National University, Seoul 08826, Korea. Electronic address: narrykim@snu.ac.kr.

摘要


During the maternal-to-zygotic transition (MZT), maternal RNAs are actively degraded and replaced by newly synthesized zygotic transcripts in a highly coordinated manner. However, it remains largely unknown how maternal mRNA decay is triggered in early vertebrate embryos. Here, through genome-wide profiling of RNA abundance and 3' modification, we show that uridylation is induced at the onset of maternal mRNA clearance. The temporal control of uridylation is conserved in vertebrates. When the homologs of terminal uridylyltransferases TUT4 and TUT7 (TUT4/7) are depleted in zebrafish and Xenopus, maternal mRNA clearance is significantly delayed, leading to developmental defects during gastrulation. Short-tailed mRNAs are selectively uridylated by TUT4/7, with the highly uridylated transcripts degraded faster during the MZT than those with unmodified poly(A) tails. Our study demonstrates that uridylation plays a crucial role in timely mRNA degradation, thereby allowing the progression of early development.

KEYWORDS: RNA decay, TAIL-seq, TUT4, TUT7, U tail, Zcchc11, Zcchc6, maternal-to-zygotic transition, poly(A) tail, uridylation

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
hour post fertilizationmorpholinosource namestrain
Zebrafish 5 hpf whole embryo injected with TUT47MO (Exp. ID: rs2)
Danio rerio GSM3024083: Zebrafish 5 hpf whole embryo injected with TUT47MO (Exp. ID: rs2); Danio rerio; RNA-Seq RNA-Seq NextSeq 500 5 TUT4 (translation-blocking), TUT7 (translation-blocking) Zebrafish whole embryo AB
Zebrafish 3 hpf whole embryo injected with TUT47MO (Exp. ID: rs2)
Danio rerio GSM3024082: Zebrafish 3 hpf whole embryo injected with TUT47MO (Exp. ID: rs2); Danio rerio; RNA-Seq RNA-Seq NextSeq 500 3 TUT4 (translation-blocking), TUT7 (translation-blocking) Zebrafish whole embryo AB
Zebrafish 5 hpf whole embryo injected with ConMO (Exp. ID: rs2)
Danio rerio GSM3024081: Zebrafish 5 hpf whole embryo injected with ConMO (Exp. ID: rs2); Danio rerio; RNA-Seq RNA-Seq NextSeq 500 5 Control Zebrafish whole embryo AB
Zebrafish 3 hpf whole embryo injected with ConMO (Exp. ID: rs2)
Danio rerio GSM3024080: Zebrafish 3 hpf whole embryo injected with ConMO (Exp. ID: rs2); Danio rerio; RNA-Seq RNA-Seq NextSeq 500 3 Control Zebrafish whole embryo AB
Zebrafish 6 hpf whole embryo injected with TUT47MO (Exp. ID: rs1b)
Danio rerio GSM3024079: Zebrafish 6 hpf whole embryo injected with TUT47MO (Exp. ID: rs1b); Danio rerio; RNA-Seq RNA-Seq NextSeq 500 6 TUT4 (translation-blocking), TUT7 (translation-blocking) Zebrafish whole embryo AB