LncRNA PTCSC3 affects drug resistance of anaplastic thyroid cancer through STAT3/INO80 pathway.

BACKGROUND:LncRNA PTCSC3 is a tumor suppressor in thyroid cancer, and its role in drug resistance of anaplastic thyroid cancer (ATC) to chemotherapy drug doxorubicin was investigated in this study. METHODS:Expression of RNA and protein was analyzed by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze the expression rate of CD133⁺ cells. The endogenous expression of related genes was modulated by recombinant plasmids and cell transfection. Combination condition and interaction between PTCSC3 and were determined by RIP and RNA pull-down assay, respectively. MTT assay was performed to detect cytotoxicity. Chromatin immunoprecipitation was conducted to identify interactions between duanyu18133 and DNA promoter of INO80. RESULTS:LncRNA PTCSC3 was low-expressed in ATC tissues and cells. Over-expressed PTCSC3 inhibited the drug resistance of ATC to doxorubicin. PTCSC3 negatively regulated and duanyu18133 promoted expression of INO80. PTCSC3 regulated INO80 through PTCSC3 suppressed stem cells properties and drug resistance of ATC to doxorubicin. CONCLUSION:LncRNA PTCSC3 inhibits INO80 expression by negatively regulating duanyu18133, and thereby attenuating drug resistance of ATC to chemotherapy drug doxorubicin.

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