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Structural changes of TasA in biofilm formation of Bacillus subtilis.

Proc. Natl. Acad. Sci. U.S.A.2018 Mar 27;115(13):3237-3242. Epub 2018 Mar 12
Anne Diehl 1 , Yvette Roske 2 , Linda Ball 1 , Anup Chowdhury 1 , Matthias Hiller 1 , Noel Molière 3 , Regina Kramer 3 , Daniel Stöppler 4 , Catherine L Worth 1 , Brigitte Schlegel 1 , Martina Leidert 1 , Nils Cremer 1 , Natalja Erdmann 1 , Daniel Lopez 5 , Heike Stephanowitz 1 , Eberhard Krause 1 , Barth-Jan van Rossum 1 , Peter Schmieder 1 , Udo Heinemann 4 , Kürşad Turgay 6 , Ümit Akbey 7 , Hartmut Oschkinat 4
Anne Diehl 1 , Yvette Roske 2 , Linda Ball 1 , Anup Chowdhury 1 , Matthias Hiller 1 , Noel Molière 3 , Regina Kramer 3 , Daniel Stöppler 4 , Catherine L Worth 1 , Brigitte Schlegel 1 , Martina Leidert 1 , Nils Cremer 1 , Natalja Erdmann 1 , Daniel Lopez 5 , Heike Stephanowitz 1 , Eberhard Krause 1 , Barth-Jan van Rossum 1 , Peter Schmieder 1 , Udo Heinemann 4 , Kürşad Turgay 6 , Ümit Akbey 7 , Hartmut Oschkinat 4
+ et al

[No authors listed]

Author information
  • 1 Leibniz-Forschungsinstitut für Molekulare Pharmakologie, 13125 Berlin, Germany.
  • 2 Max-Delbrück-Center for Molecular Medicine, 13125 Berlin, Germany.
  • 3 Institut für Mikrobiologie, Leibniz Universität Hannover, 30419 Hannover, Germany.
  • 4 Institut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, Germany.
  • 5 Centro Nacional de Biotecnologia, Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain.
  • 6 Institut für Mikrobiologie, Leibniz Universität Hannover, 30419 Hannover, Germany; heinemann@mdc-berlin.de turgay@ifmb.uni-hannover.de oschkinat@fmp-berlin.de.
  • 7 Aarhus Institute of Advanced Studies, Aarhus University, 8000 Aarhus C, Denmark.

摘要


Microorganisms form surface-attached communities, termed biofilms, which can serve as protection against host immune reactions or antibiotics. Bacillus subtilis biofilms contain TasA as major proteinaceous component in addition to exopolysaccharides. In stark contrast to the initially unfolded biofilm proteins of other bacteria, TasA is a soluble, stably folded monomer, whose structure we have determined by X-ray crystallography. Subsequently, we characterized in vitro different oligomeric forms of TasA by NMR, EM, X-ray diffraction, and analytical ultracentrifugation (AUC) experiments. However, by magic-angle spinning (MAS) NMR on live biofilms, a swift structural change toward only one of these forms, consisting of homogeneous and protease-resistant, β-sheet-rich fibrils, was observed in vivo. Thereby, we characterize a structural change from a globular state to a fibrillar form in a functional prokaryotic system on the molecular level.

KEYWORDS: Bacillus subtilis, NMR, TasA, biofilm, structure