Blocking the ART2.2/P2X7-system is essential to avoid a detrimental bias in functional CD4 TÂ cell studies.

Eur. J. Immunol.2018 Jun;48(6):1078-1081. doi:10.1002/eji.201747420. Epub 2018 Mar 23

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Murine TÂ cell subsets differ in their expression level of P2X7. Depending on several parameters like extracellular NAD⁺ , P2X7 can be ADP-ribosylated rapidly by adjacent ARTC2.2 resulting in susceptibilities to apoptosis to a varying extent. This detrimental effect can be prevented when drugs like KN-62 are present during cell preparations.

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Blocking the ART2.2/P2X7-system is essential to avoid a detrimental bias in functional CD4 TÂ cell studies.

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