[No authors listed]
It has been previously reported that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC)âexosomes exhibit cardioprotective effects on the rat acute myocardial infarction (AMI) models and cardiomyocyte hypoxia injury models in vitro, however the exact mechanisms involved require further investigation. The present study aimed to investigate the repair effects of hucMSCâexosomes on myocardial injury via the regulation of mothers against decapentaplegic homolog 7 (Smad7) expression. Compared with sham or normoxia groups (in vivo and in vitro, respectively), western blotting demonstrated that Smad7 expression was significantly decreased in the borderline area of infraction myocardium and in H9C2(2â1) cells following hypoxiaâinduced injury. Additionally, microRNA (miR)â125bâ5p expression was markedly increased using reverse transcriptionâquantitative polymerase chain reaction, but was reversed by hucMSCâexosomes. Trypan blue staining and lactate dehydrogenase release detection demonstrated that cell injury was significantly increased in the AMI + PBS and hypoxia group compared with in the sham and normoxia groups and was inhibited by hucMSCâexosomes. A dual luciferase reporter gene assay confirmed that Smad7 is a target gene of miRâ125bâ5p. In addition, miRâ125bâ5p mimics promoted H9C2(2â1) cell injury following 48 h exposure to hypoxia. Downregulation of Smad7 expression under hypoxia was increased by miRâ125bâ5p mimics compared with the mimic negative control, and hucMSCâexosomes partially alleviated this phenomenon. In conclusion, hucMSCâexosomes may promote Smad7 expression by inhibiting miRâ125bâ5p to increase myocardial repair. The present study may provide a potential therapeutic approach to improve myocardial repair following AMI.
KEYWORDS: {{ getKeywords(articleDetailText.words) }}
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
{{attr}} | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
{{ dataList.sampleTitle }} | {{ dataList.organism }} | {{ dataList.expermentTitle }} | {{ dataList.sampleType }} | {{ dataList.libraryInstrument }} | {{ showAttributeName(index,attr,dataList.attributes) }} |
{{ list.authorName }} {{ list.authorName }} |