TMCO1 is essential for ovarian follicle development by regulating ER Ca²⁺ store of granulosa cells.

TMCO1 (transmembrane and coiled-coil domains 1) is an endoplasmic reticulum (ER) transmembrane protein that actively prevents Ca²⁺ stores from overfilling. To characterize its physiological function(s), we generated Tmco1^-/- knockout (KO) mice. In addition to the main clinical features of human cerebrofaciothoracic (CFT) dysplasia spectrum, Tmco1^-/- females manifest gradual loss of ovarian follicles, impaired ovarian follicle development, and subfertility with a phenotype analogous to the premature ovarian failure (POF) in women. In line with the role of TMCO1 as a Ca²⁺ load-activated Ca²⁺ channel, we have detected a supernormal Ca²⁺ signaling in Tmco1^-/- granulosa cells (GCs). Interestingly, although spontaneous Ca²⁺ oscillation pattern was altered, ER Ca²⁺ stores of germinal vesicle (GV) stage oocytes and metaphase II (MII) arrested eggs were normal upon Tmco1 ablation. Combined with RNA-sequencing analysis, we also detected increased ER stress-mediated apoptosis and enhanced reactive oxygen species level in Tmco1^-/- GCs, indicating the dysfunctions of GCs upon TMCO1 deficiency. Taken together, these results reveal that TMCO1 is essential for ovarian follicle development and female fertility by maintaining ER Ca²⁺ homeostasis of GCs, disruption of which causes ER stress-mediated apoptosis and increased cellular level in GCs and thus leads to impaired ovarian follicle development.

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