Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins.
Mol. Biol. Cell. 2018 Apr 15;29(8):937-947. Epub 2018 Mar 30
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摘要
Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.
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基因功能
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原始数据
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文献解读
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