Rapid IFM Dissection for Visualizing Fluorescently Tagged Sarcomeric Proteins.
Bio Protoc. 2017 Nov 20;7(22). doi:10.21769/BioProtoc.2606
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摘要
Sarcomeres, the smallest contractile unit of muscles, are arguably the most impressive actomyosin structure. Yet a complete understanding of sarcomere formation and maintenance is missing. The Drosophila indirect flight muscle (IFM) has proven to be a very valuable model to study sarcomeres. Here, we present a protocol for the rapid dissection of IFM and analysis of sarcomeres using fluorescently tagged proteins.
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文献解读
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