Effect of Reprimo Down-regulation on Malignant Transformation of Intraductal Papillary Mucinous Neoplasm.

OBJECTIVES:Reprimo gene is a cytoplasmic protein belonging to a family of molecules controlled by p53 that inhibits cell cycle progression. Ectopic expression of Reprimo results in cell cycle arrest at the G2 phase. The aim of this study was to investigate the impact of Reprimo expression on tumorigenesis of intraductal papillary mucinous neoplasm surgical cases of were collected retrospectively. Twenty-eight patients had benign (low-grade dysplasia, n = 18; intermediate-grade dysplasia, n = 10), and the remaining 9 had malignant Iduanyu1451s (high-grade dysplasia, n = 4; invasive carcinoma, n = 5). DNA from tumor samples was extracted. DNA methylation patterns of Reprimo were determined by the methods of methylation-specific polymerase chain reaction and immunohistochemistry. The methylation status of Reprimo was compared between benign Iduanyu1451s and malignant incidence of aberrant DNA methylation of Reprimo was significantly higher in malignant Iduanyu1451s than in benign Iduanyu1451s (78% vs 32%, P = 0.016). Furthermore, the incidence of immunohistochemical Reprimo expression was significantly lower in malignant Iduanyu1451s than in benign Iduanyu1451s (22% vs 82%, P = 0.002). CONCLUSIONS:Reprimo methylation was found more frequently in malignant Reprimo methylation is involved in malignant transformation of

KEYWORDS: {{ getKeywords(articleDetailText.words) }}