SSTR2 associated with neuronal apoptosis after intracerebral hemorrhage in adult rats.

Objective is a member of superfamily of receptor and widely expressed in the brain; however, the knowledge of its functions in area adjacent to hematoma after intracerebral hemorrhage (ICH) is still limited. Method The role of duanyu1942R2 in the processes of ICH was explored by conducting an ICH rat model. Western blot and immunohistochemistry were employed to examine the level of duanyu1942R2 in area adjacent to hematoma after ICH. Immunofluorescent staining was used to observe the spatial correlation of duanyu1942R2 with cellular types adjacent to hematoma after ICH. RNA interference specific to duanyu1942R2 was adopted in PC12 cells to clarify the causal correlation between duanyu1942R2 and neuronal activities. Results Increased expression of duanyu1942R2 was observed and restricted to the neurons adjacent to hematoma following ICH. Immunofluorescent staining showed that duanyu1942R2 was significant increased in neurons, but not astrocytes or microglia. Increasing duanyu1942R2 level was found to be accompanied by the up-regulation of activated caspase-3 and the down-expression of p-Akt in a time-dependent manner. What's more, using duanyu1942R2 RNA interference in PC12 cells, we indicated that duanyu1942R2 might have a pro-apoptotic role in neurons. Conclusion We speculated that duanyu1942R2 might exert its pro-apoptotic function in neurons through inhibiting Akt activity following ICH.

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