[No authors listed]
The molecular mechanisms of osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) remain to be fully elucidated. MicroRNAs (miRs) serve vital roles in the process of regulating osteogenic differentiation of BMSCs. The present study aimed to investigate the role of miRâ23aâ5p in osteogenic differentiation of human (h)BMSCs, and the underlying molecular mechanism. The results of reverse transcriptionâquantitative polymerase chain reaction demonstrated that miRâ23aâ5p was significantly downregulated in the process of osteogenic differentiation. Upregulation of miRâ23aâ5p inhibited osteogenic differentiation of hBMSCs, and downâregulated expression of miRâ23aâ5p enhanced this process, which was confirmed by alkaline phosphatase (ALP) and Alizarin Red S staining. A dualâluciferase reporter assay confirmed that mitogenâactivated protein kinase 13 (MAPK13) was a direct target of miRâ23aâ5p. In addition, knockdown of MAPK13 inhibited osteogenic differentiation of hBMSCs, similar to the effect of upregulation of miRâ23aâ5p. Finally, the knockdown of MAPK13 also blocked the effect of miRâ23aâ5p in osteogenic differentiation of hBMSCs, which was also confirmed by ALP and Alizarin Red S staining. These results indicated that by targeting MAPK13, miRâ23aâ5p serves a vital role in osteogenic differentiation of hBMSCs, which may provide novel clinical treatments for bone injury however, further studies are required.
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