RMR (Receptor Membrane RING-H2) type 1 and 2 show different promoter activities and subcellular localizations in Arabidopsis thaliana.

Soluble vacuolar proteins reach their compartments of final accumulation through the binding with specific transmembrane cargo receptors. In Arabidopsis thaliana two different families of receptors have been characterized. The AtVSRs (Vacuolar Sorting Receptor), which are known to be involved in the protein sorting to lytic vacuoles (LV), and the (Receptor Membrane RING-H2), for which there is less evidence for a role in the traffic to the protein storage vacuole (PSV). In this study we investigated the localization and tissue expression of two and 2) in their species of origin, A. thaliana. Our experiments using leaf protoplasts and transgenic plants supported previous results of subcellular localization in Nicotiana benthamiana that visualized and 2 in the cisternae of endoplasmic reticulum (ER) and in the trans-Golgi network (TGN), respectively. The promoter activities of Atduanyu17481 and detected in transgenic A. thaliana lines suggest that the expression of these two receptors only partially overlap in some organs and tissues. These results suggest that Atduanyu17481 and 2 are not functionally redundant, but could also interact and participate in the same cellular process in tissues with an overlapping expression.

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