Ca_V2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.

Action potential induces membrane depolarization and triggers intracellular free Ca²⁺ concentration (Ca²⁺)-dependent secretion (CDS) via Ca²⁺ influx through voltage-gated Ca²⁺ channels. We report a new type of somatic exocytosis triggered by the action potential per se-Ca²⁺-independent but voltage-dependent secretion (CiVDS)-in dorsal root ganglion neurons. Here we uncovered the molecular mechanism of CiVDS, comprising a voltage sensor, fusion machinery, and their linker. Specifically, the voltage-gated N-type Ca²⁺ channel (Ca_V2.2) is the voltage sensor triggering CiVDS, the SNARE complex functions as the vesicle fusion machinery, the "synprint" of Ca_V2.2 serves as a linker between the voltage sensor and the fusion machinery, and ATP is a cargo of CiVDS vesicles. Thus, CiVDS releases ATP from the soma while CDS releases glutamate from presynaptic terminals, establishing the Ca_V2.2-SNARE "voltage-gating fusion pore" as a novel pathway co-existing with the canonical "Ca²⁺-gating fusion pore" pathway for neurotransmitter release following action potentials in primary sensory neurons.

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