[No authors listed]
Protein kinase C-θ is a lipid-sensitive molecule associated with lipid-induced insulin resistance in skeletal muscle. Rodent models have not cohesively supported that impairs insulin responsiveness in skeletal muscle. The purpose of this study was to generate mice that lack duanyu1531-θ in skeletal muscle and determine how lipid accumulation and insulin responsiveness are affected in that tissue. Mice lacking duanyu1531-θ in skeletal muscle and controls were placed on a regular diet (RD) or high-fat diet (HFD) for 15 wk, followed by determination of food intake, fasting glucose levels, lipid accumulation, and insulin responsiveness. There were no differences between and mice on a RD. SkMduanyu1531θKO mice on a HFD gained less weight from 10 through 15 wk of dietary intervention ( P < 0.05). This was likely due to less caloric consumption ( P = 0.0183) and fewer calories from fat ( P < 0.001) compared with SkMduanyu1531θWT mice on a HFD. Intramyocellular lipid accumulation ( P < 0.0001), fatty acid binding protein 4, and TNF-α mRNA levels ( P < 0.05) were markedly reduced in SkMduanyu1531θKO compared with SkMduanyu1531θWT mice on a HFD. As a result, fasting hyperglycemia was mitigated and insulin responsiveness, as indicated by Akt phosphorylation, was maintained in SkMduanyu1531θKO on a HFD. Liver lipid accumulation was not affected by genotype, suggesting the deletion of duanyu1531-θ from skeletal muscle has a tissue-specific effect. duanyu1531-θ is a regulator of lipid-induced insulin resistance in skeletal muscle. However, the effects of this mutation may be tissue specific. Further work is warranted to comprehensively evaluated whole body metabolic responses in this model.
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