Epithelial-mesenchymal interactions are crucial for the development of numerous animal structures. Thus, unraveling how molecular tools are recruited in different lineages to control interplays between these tissues is key to understanding morphogenetic evolution. Here, we study Esrp genes, which regulate extensive splicing programs and are essential for mammalian organogenesis. We find that Esrp homologs have been independently recruited for the development of multiple structures across deuterostomes. Although Esrp is involved in a wide variety of ontogenetic processes, our results suggest ancient roles in non-neural ectoderm and regulating specific mesenchymal-to-epithelial transitions in deuterostome ancestors. However, consistent with the extensive rewiring of Esrp-dependent splicing programs between phyla, most developmental defects observed in vertebrate mutants are related to other types of morphogenetic processes. This is likely connected to the origin of an event in Fgfr, which was recruited as an Esrp target in stem chordates and subsequently co-opted into the development of many novel traits in vertebrates.
Sample name | Organism | Experiment title | Sample type | Library instrument | Attributes | |||||||||||||||||||||||||||||||||||||||||||||||||
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genotype | source name | |||||||||||||||||||||||||||||||||||||||||||||||||||||
Esrp SplMO knockdown 24hpf sea urchin embryos replicate 2 run 1 | Strongylocentrotus purpuratus | GSM2560400: Esrp SplMO knockdown 24hpf sea urchin embryos replicate 2 run 1; Strongylocentrotus purpuratus; RNA-Seq | RNA-Seq | Illumina HiSeq 2500 | Esrp KD | Purple sea urchin embryos 24hpf | ||||||||||||||||||||||||||||||||||||||||||||||||
Esrp SplMO knockdown 24hpf sea urchin embryos replicate 1 run 1 | Strongylocentrotus purpuratus | GSM2560399: Esrp SplMO knockdown 24hpf sea urchin embryos replicate 1 run 1; Strongylocentrotus purpuratus; RNA-Seq | RNA-Seq | Illumina HiSeq 2500 | Esrp KD | Purple sea urchin embryos 24hpf | ||||||||||||||||||||||||||||||||||||||||||||||||
Control 24hpf sea urchin embryos replicate 2 run 1 | Strongylocentrotus purpuratus | GSM2560398: Control 24hpf sea urchin embryos replicate 2 run 1; Strongylocentrotus purpuratus; RNA-Seq | RNA-Seq | Illumina HiSeq 2500 | Wild type | Purple sea urchin embryos 24hpf | ||||||||||||||||||||||||||||||||||||||||||||||||
Control 24hpf sea urchin embryos replicate 1 run 1 | Strongylocentrotus purpuratus | GSM2560397: Control 24hpf sea urchin embryos replicate 1 run 1; Strongylocentrotus purpuratus; RNA-Seq | RNA-Seq | Illumina HiSeq 2500 | Wild type | Purple sea urchin embryos 24hpf | ||||||||||||||||||||||||||||||||||||||||||||||||
Esrp1 & 2 DKO 5dpf zebrafish embryos replicate 2 run 1 | Danio rerio | GSM2560396: Esrp1 & 2 DKO 5dpf zebrafish embryos replicate 2 run 1; Danio rerio; RNA-Seq | RNA-Seq | Illumina HiSeq 2500 | Esrp1 & Esrp2 KO | Zebrafish whole embryos 5dpf |