例如:"lncRNA", "apoptosis", "WRKY"

Evolutionary recruitment of flexible Esrp-dependent splicing programs into diverse embryonic morphogenetic processes.

Nat Commun. 2017 Nov 27;8(1):1799
Demian Burguera 1 , Yamile Marquez 1 , Claudia Racioppi 2 , Jon Permanyer 1 , Antonio Torres-Méndez 1 , Rosaria Esposito 3 , Beatriz Albuixech-Crespo 4 , Lucía Fanlo 5 , Ylenia D'Agostino 3 , Andre Gohr 1 , Enrique Navas-Perez 4 , Ana Riesgo 6 , Claudia Cuomo 3 , Giovanna Benvenuto 3 , Lionel A Christiaen 2 , Elisa Martí 5 , Salvatore D'Aniello 3 , Antonietta Spagnuolo 3 , Filomena Ristoratore 3 , Maria Ina Arnone 7 , Jordi Garcia-Fernàndez 8 , Manuel Irimia 9
Demian Burguera 1 , Yamile Marquez 1 , Claudia Racioppi 2 , Jon Permanyer 1 , Antonio Torres-Méndez 1 , Rosaria Esposito 3 , Beatriz Albuixech-Crespo 4 , Lucía Fanlo 5 , Ylenia D'Agostino 3 , Andre Gohr 1 , Enrique Navas-Perez 4 , Ana Riesgo 6 , Claudia Cuomo 3 , Giovanna Benvenuto 3 , Lionel A Christiaen 2 , Elisa Martí 5 , Salvatore D'Aniello 3 , Antonietta Spagnuolo 3 , Filomena Ristoratore 3 , Maria Ina Arnone 7 , Jordi Garcia-Fernàndez 8 , Manuel Irimia 9
+ et al

[No authors listed]

Author information
  • 1 Universitat Pompeu Fabra (UPF), Barcelona, 08003, Spain.
  • 2 Center for Developmental Genetics, Department of Biology, New York University, New York, NY, 1003, USA.
  • 3 Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy.
  • 4 Department of Genetics, School of Biology, and Institut de Biomedicina (IBUB), University of Barcelona, Diagonal 643, Barcelona, 08028, Spain.
  • 5 Instituto de Biología Molecular de Barcelona, CSIC, Parc Científic de Barcelona, Baldiri Reixac 20, Barcelona, 08028, Spain.
  • 6 Department of Life Sciences, Natural History Museum of London, Cromwell Road, SW7 5BD, London, UK.
  • 7 Stazione Zoologica Anton Dohrn, Villa Comunale, 80121, Napoli, Italy. miarnone@szn.it.
  • 8 Department of Genetics, School of Biology, and Institut de Biomedicina (IBUB), University of Barcelona, Diagonal 643, Barcelona, 08028, Spain. jordigarcia@ub.edu.
  • 9 Universitat Pompeu Fabra (UPF), Barcelona, 08003, Spain. mirimia@gmail.com.

摘要


Epithelial-mesenchymal interactions are crucial for the development of numerous animal structures. Thus, unraveling how molecular tools are recruited in different lineages to control interplays between these tissues is key to understanding morphogenetic evolution. Here, we study Esrp genes, which regulate extensive splicing programs and are essential for mammalian organogenesis. We find that Esrp homologs have been independently recruited for the development of multiple structures across deuterostomes. Although Esrp is involved in a wide variety of ontogenetic processes, our results suggest ancient roles in non-neural ectoderm and regulating specific mesenchymal-to-epithelial transitions in deuterostome ancestors. However, consistent with the extensive rewiring of Esrp-dependent splicing programs between phyla, most developmental defects observed in vertebrate mutants are related to other types of morphogenetic processes. This is likely connected to the origin of an event in Fgfr, which was recruited as an Esrp target in stem chordates and subsequently co-opted into the development of many novel traits in vertebrates.

原始数据


 保存测序数据
Sample name
Organism Experiment title Sample type Library instrument Attributes
genotypesource name
Esrp SplMO knockdown 24hpf sea urchin embryos replicate 2 run 1
Strongylocentrotus purpuratus GSM2560400: Esrp SplMO knockdown 24hpf sea urchin embryos replicate 2 run 1; Strongylocentrotus purpuratus; RNA-Seq RNA-Seq Illumina HiSeq 2500 Esrp KD Purple sea urchin embryos 24hpf
Esrp SplMO knockdown 24hpf sea urchin embryos replicate 1 run 1
Strongylocentrotus purpuratus GSM2560399: Esrp SplMO knockdown 24hpf sea urchin embryos replicate 1 run 1; Strongylocentrotus purpuratus; RNA-Seq RNA-Seq Illumina HiSeq 2500 Esrp KD Purple sea urchin embryos 24hpf
Control 24hpf sea urchin embryos replicate 2 run 1
Strongylocentrotus purpuratus GSM2560398: Control 24hpf sea urchin embryos replicate 2 run 1; Strongylocentrotus purpuratus; RNA-Seq RNA-Seq Illumina HiSeq 2500 Wild type Purple sea urchin embryos 24hpf
Control 24hpf sea urchin embryos replicate 1 run 1
Strongylocentrotus purpuratus GSM2560397: Control 24hpf sea urchin embryos replicate 1 run 1; Strongylocentrotus purpuratus; RNA-Seq RNA-Seq Illumina HiSeq 2500 Wild type Purple sea urchin embryos 24hpf
Esrp1 & 2 DKO 5dpf zebrafish embryos replicate 2 run 1
Danio rerio GSM2560396: Esrp1 & 2 DKO 5dpf zebrafish embryos replicate 2 run 1; Danio rerio; RNA-Seq RNA-Seq Illumina HiSeq 2500 Esrp1 & Esrp2 KO Zebrafish whole embryos 5dpf