Calpain-5 gene expression in the mouse eye and brain.

BMC Res Notes. 2017 Nov 21;10(1):602

Kellie Schaefer ¹ , MaryAnn Mahajan ¹ , Anuradha Gore ¹ , Stephen H Tsang ² , Alexander G Bassuk ³ ,

Kellie Schaefer ¹ , MaryAnn Mahajan ¹ , Anuradha Gore ¹ , Stephen H Tsang ² , Alexander G Bassuk ³ , Vinit B Mahajan ⁴

+ et al

Author information

¹ Omics Laboratory, Department of Ophthalmology, Byers Eye Institute, Stanford University, Palo Alto, CA, 94304, USA.
² Bernard and Shirlee Brown Glaucoma Laboratory, Department of Pathology and Cell Biology, Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
³ Department of Pediatrics, University of Iowa, Iowa City, IA, USA.
⁴ Palo Alto Veterans Administration, Palo Alto, CA, USA. vinit.mahajan@stanford.edu.

摘要

OBJECTIVE:Our objective was to characterize CAPN5 gene expression in the mouse central nervous system. Mouse brain and eye sections were probed with two high-affinity RNA oligonucleotide analogs designed to bind CAPN5 RNA and one scramble, control oligonucleotide. Images were captured in brightfield. RESULTS:CAPN5 RNA probes were validated on mouse breast cancer tumor tissue. In the eye, CAPN5 was expressed in the ganglion cell, inner nuclear and outer nuclear layers of the retina. Signal could not be detected in the ciliary body or the iris because of the high density of melanin. In the brain, CAPN5 was expressed in the granule cell layers of the hippocampus and cerebellum. There was scattered expression in pons. The visual cortex showed faint signal. Most signal in the brain was in a punctate pattern.

KEYWORDS: Brain, CAPN5, Calpain, Gene expression, In situ hybridization, Retina

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