C6 glioma-conditioned medium induces malignant transformation of mesenchymal stem cells: Possible role of S100B/RAGE pathway.

Mesenchymal stem cells (MSCs) have been widely studied as an attractive therapeutic agent for the treatment of tumors. However, the adverse effects of the tumor paracrine factors who affect MSCs are still unclear. In this study, we report for the first time that C6 glioma-conditioned medium (GCM) induces malignant transformation of MSCs. In contrast to MSCs, the transformed mesenchymal stem cells (TMCs) exhibited tumor cell characterizations in vitro and highly tumorigenic in vivo. Furthermore, GCM and recombinant S100B increased receptor for advanced glycation end products and its downstream Akt1, genes expression as well as phosphorylation and transcriptional activation. Finally, blockage of interaction by inhibitor FPS-ZM1 attenuated GCM and S100B-induced Akt1, duanyu18133 activation, abolished its cell proliferation, migration and invasion actions. Together, these results suggest that the duanyu1648 pathway may play a possible role in malignant transformation procedure of MSCs, and that this process may be mediated through S100B.

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